AmScope IN300 Series Inverted Phase-Contrast Biological EPI-Fluorescent Trinocular Compound Microscope 40X-1000X Magnification with 30W Halogen
Key Features
- 40X to 1000X expanded magnification range using a quadruple lens turret and additional 25X eyepieces for seven unique settings.
- An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
- The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
- The infinity-corrected optical system ensures high-quality, high-resolution images.
- Specialized 10X and 20X objective lenses are included for phase-contrast applications.
- The trinocular photo port provides a dedicated interface for mounting a camera.
The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 25X eyepieces expand the maximum magnification to 1000X with 7 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.
Transmitted Illumination
The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Phase Contrast
The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.
Epi-fluorescence Illumination
For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.
Fluorescence
Filters | ||||||||||||
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Mercury HBO Excitation | ||||||||||||
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
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Specifications
Optical System | Infinity-corrected | ||||
Head | Trinocular, 45° incline | ||||
Interpupillary Adjustment | Siedentopf compensation-free gemel, 50mm-75mm | ||||
Ocular Diameter | 30mm | ||||
Ocular Diopters | right ocular | ||||
Eyepieces | 10X, 25X | ||||
Photo-port | height-adjustable 23mm tube | ||||
Objective Lenses | Infinity-corrected | ||||
Objective Turret | Quadruple | ||||
Focusing System |
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Stage | fixed-height, mechanical X-Y translation | ||||
Stage Dimensions | 172mm x 242mm | ||||
Stage X-Y Travel Range | 75mm x 40mm | ||||
Specimen Holders | Terasaki plate, Petri dish | ||||
Episcopic Illumination (Fluorescence) | 100W mercury HBO arc-lamp, centerable | ||||
Transmitted Illumination | 12V 30W halogen variable intensity |
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Condenser | NA 0.3, iris diaphragm, filter slot | ||||
Power | 110VAC 60Hz | ||||
Objective Lenses
Magnification | Corrections | NA | Working Distance | Cover-glass Thickness |
---|---|---|---|---|
4X | plan fluor | 0.15 | 28mm | 1.5mm |
10X | plan fluor, phase-contrast | 0.30 | 3mm | 1.5mm |
20X | plan fluor, phase-contrast | 0.50 | 4.27mm | 1.5mm |
40X | plan fluor | 0.60 | 2.23mm | 1.5mm |
Packing List:
The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 25X eyepieces expand the maximum magnification to 1000X with 7 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.
Transmitted Illumination
The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Phase Contrast
The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.
Epi-fluorescence Illumination
For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.
Fluorescence
Filters | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||
Mercury HBO Excitation | ||||||||||||
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
|
Specifications
Optical System | Infinity-corrected | ||||
Head | Trinocular, 45° incline | ||||
Interpupillary Adjustment | Siedentopf compensation-free gemel, 50mm-75mm | ||||
Ocular Diameter | 30mm | ||||
Ocular Diopters | right ocular | ||||
Eyepieces | 10X, 25X | ||||
Photo-port | height-adjustable 23mm tube | ||||
Objective Lenses | Infinity-corrected | ||||
Objective Turret | Quadruple | ||||
Focusing System |
|
||||
Stage | fixed-height, mechanical X-Y translation | ||||
Stage Dimensions | 172mm x 242mm | ||||
Stage X-Y Travel Range | 75mm x 40mm | ||||
Specimen Holders | Terasaki plate, Petri dish | ||||
Episcopic Illumination (Fluorescence) | 100W mercury HBO arc-lamp, centerable | ||||
Transmitted Illumination | 12V 30W halogen variable intensity |
||||
Condenser | NA 0.3, iris diaphragm, filter slot | ||||
Power | 110VAC 60Hz | ||||
Objective Lenses
Magnification | Corrections | NA | Working Distance | Cover-glass Thickness |
---|---|---|---|---|
4X | plan fluor | 0.15 | 28mm | 1.5mm |
10X | plan fluor, phase-contrast | 0.30 | 3mm | 1.5mm |
20X | plan fluor, phase-contrast | 0.50 | 4.27mm | 1.5mm |
40X | plan fluor | 0.60 | 2.23mm | 1.5mm |